Awesome Open Source
Awesome Open Source
Table of content

Table of content



Some biology

If you are from fields outside of biology, places to get you started:

Some statistics

linear algebra

Bayesian Statistics

Learning Latex

Linux commands

Theory and quick reference

There are 3 file descriptors, stdin, stdout and stderr (std=standard).

Basically you can:

redirect stdout to a file redirect stderr to a file redirect stdout to a stderr redirect stderr to a stdout redirect stderr and stdout to a file redirect stderr and stdout to stdout redirect stderr and stdout to stderr 1 'represents' stdout and 2 stderr. A little note for seeing this things: with the less command you can view both stdout (which will remain on the buffer) and the stderr that will be printed on the screen, but erased as you try to 'browse' the buffer.

  • stdout 2 file

This will cause the ouput of a program to be written to a file.

     ls -l > ls-l.txt

Here, a file called 'ls-l.txt' will be created and it will contain what you would see on the screen if you type the command 'ls -l' and execute it.

  • stderr 2 file

This will cause the stderr ouput of a program to be written to a file.

     grep da * 2> grep-errors.txt

Here, a file called 'grep-errors.txt' will be created and it will contain what you would see the stderr portion of the output of the 'grep da *' command.

  • stdout 2 stderr

This will cause the stderr ouput of a program to be written to the same filedescriptor than stdout.

     grep da * 1>&2

Here, the stdout portion of the command is sent to stderr, you may notice that in differen ways.

  • stderr 2 stdout

This will cause the stderr ouput of a program to be written to the same filedescriptor than stdout.

     grep * 2>&1

Here, the stderr portion of the command is sent to stdout, if you pipe to less, you'll see that lines that normally 'dissapear' (as they are written to stderr) are being kept now (because they're on stdout).

  • stderr and stdout 2 file

This will place every output of a program to a file. This is suitable sometimes for cron entries, if you want a command to pass in absolute silence.

     rm -f $(find / -name core) &> /dev/null

This (thinking on the cron entry) will delete every file called 'core' in any directory. Notice that you should be pretty sure of what a command is doing if you are going to wipe it's output.

  • change permissions of files
    each digit is for: user, group and other.

chmod 754 myfile: this means the user has read, write and execute permssion; member in the same group has read and execute permission but no write permission; other people in the world only has read permission.

4 stands for "read",
2 stands for "write",
1 stands for "execute", and
0 stands for "no permission."
So 7 is the combination of permissions 4+2+1 (read, write, and execute), 5 is 4+0+1 (read, no write, and execute), and 4 is 4+0+0 (read, no write, and no execute).

It is sometimes hard to remember. one can use the letter:The letters u, g, and o stand for "user", "group", and "other"; "r", "w", and "x" stand for "read", "write", and "execute", respectively.

chmod u+x myfile
chmod g+r myfile

Do not give me excel files!

How to name files

It is really important to name your files correctly! see a ppt by Jenny Bryan.

Three principles for (file) names:

  • Machine readable (do not put special characters and space in the name)
  • Human readable (Easy to figure out what the heck something is, based on its name, add slug)
  • Plays well with default ordering:
  1. Put something numeric first

  2. Use the ISO 8601 standard for dates (YYYY-MM-DD)

  3. Left pad other numbers with zeros

If you have to rename the files...

  • brename A cross-platform command-line tool for safely batch renaming files/directories via regular expression (supporting Windows, Linux and OS X) from ShenWei is very useful!

Good naming of your files can help you to extract meta data from the file name

  • dirdf Create tidy data frames of file metadata from directory and file names.
> dir("examples/dataset_1/")
[1] "2013-06-26_BRAFWTNEG_Plasmid-Cellline-100_A01.csv"
[2] "2013-06-26_BRAFWTNEG_Plasmid-Cellline-100_A02.csv"
[3] "2014-02-26_BRAFWTNEG_FFPEDNA-CRC-1-41_D08.csv"
[4] "2014-03-05_BRAFWTNEG_FFPEDNA-CRC-REPEAT_H03.csv"
[5] "2016-04-01_BRAFWTNEG_FFPEDNA-CRC-1-41_E12.csv"

> library("dirdf")
> dirdf("examples/dataset_1/", template="date_assay_experiment_well.ext")
        date     assay           experiment well ext                                          pathname
1 2013-06-26 BRAFWTNEG Plasmid-Cellline-100  A01 csv 2013-06-26_BRAFWTNEG_Plasmid-Cellline-100_A01.csv
2 2013-06-26 BRAFWTNEG Plasmid-Cellline-100  A02 csv 2013-06-26_BRAFWTNEG_Plasmid-Cellline-100_A02.csv
3 2014-02-26 BRAFWTNEG     FFPEDNA-CRC-1-41  D08 csv     2014-02-26_BRAFWTNEG_FFPEDNA-CRC-1-41_D08.csv


Using these tool will greatly improve your working efficiency and get rid of most of your for loops.

  1. xargs
  2. GNU parallel. one of my post here
  3. gxargs by Brent Pedersen. Written in GO.
  4. rush A cross-platform command-line tool for executing jobs in parallel by Shen Wei. I use his other tools such as brename and csvtk.
  5. future: Unified Parallel and Distributed Processing in R for Everyone
  6. furrr Apply Mapping Functions in Parallel using Futures


Data transfer

a blog post by Mark Ziemann


updating R

# Install new version of R (lets say 3.5.0 in this example)

# Create a new directory for the version of R

# Re-start R so the .libPaths are updated

# Lookup what packages were in your old package library
pkgs <- fs::dirname(fs::dir_ls("~/Library/R/3.4/library"))

# Filter these packages as needed

# Install the packages in the new version

Better R code

Shiny App

  • [Omicsplayground[]
  • A Framework for Building Robust Shiny Apps golem
  • [bootstrapllib}( Tools for styling shiny and rmarkdown from R via Bootstrap (3 or 4) Sass
  • The Shiny AWS Book How to set up Shiny in AWS
  • imolaBridging the gap between R/shiny and CSS layouts (grid and flexbox)!

profile R code

  • profvis Interactive Visualizations for Profiling R Code.
  • proffer The proffer package profiles R code to find bottlenecks.
  • rco - The R Code Optimizer Make your R code run faster! rco analyzes your code and applies different optimization strategies that return an R code that runs faster.

R tools for data wrangling, tidying and visualizing.

If you already know the mapping in advance (like the above example) you should use the .data pronoun from rlang to make it explicit that you are referring to the drv in the layer data and not some other variable named drv (which may or may not exist elsewhere). To avoid a similar note from the CMD check about .data, use #' @importFrom rlang .data in any roxygen code block (typically this should be in the package documentation as generated by usethis::use_package_doc()).

  • If you know the mapping or facet specification is col in advance, use aes(.data$col) or vars(.data$col).
  • If col is a variable that contains the column name as a character vector, use aes(.data[[col]] or vars(.data[[col]]).
  • If you would like the behaviour of col to look and feel like it would within aes() and vars(), use aes({{ col }}) or vars({{ col }}).

Genomic data visulization

  • karyoploteR Really powerful and versatile tool.
  • Bentobox BentoBox empowers users to programmatically and flexibly generate multi-panel figures.

Sankey graph

Handling big data in R

Write your own R package


  • This is a must read for writing good documentations: A blog post. I saved it to a pdf and uploaded to this repo.

handling arguments at the command line

visualization in general


python tips and tools

machine learning

Amazon cloud computing

Intro to AWS Cloud Computing


There are many online web based tools for visualization of (cancer) genomic data. I put my collections here. I use R for visulization. see a nice post by using python by Radhouane Aniba:Genomic Data Visualization in Python

  • UCSC cancer genome browser It has many data including TCGA data buit in, and can be very handy for both bench scientist and bioinformaticians.
  • UCSC Xena. A new tool developed by UCSC team as well. Poteintially very useful, but need more tutorials to follow.
  • UCSC genome browser. One of the most famous genome browser and my favoriate. Every person studying genetics, genomics and molecular biology needs to know how to use it. Tutorials from OpenHelix.
  • Epiviz 3 is an interactive visualization tool for functional genomics data. It supports genome navigation like other genome browsers, but allows multiple visualizations of data within genomic regions using scatterplots, heatmaps and other user-supplied visualizations.
  • Mutation Annotation & Genome Interpretation TCGA: MAGA
  • GeneProteinViz (GPViz) is a versatile Java-based software for dynamic gene-centered visualization of genomic regions and/or variants.
  • ProteinPaint: Web Application for Visualizing Genomic Data The software developed for this project highlights critical attributes about the mutations, including the form of protein variant (e.g. the new amino acid as a result of missense mutation), the name of sample from which the mutation was identified, whether the mutation is somatic or germline,


Large data consortium data mining

Integrative analysis

Interactive visualization


<script async src="//" charset="utf-8"></script>

paper: Outlier Preservation by Dimensionality Reduction Techniques

"MDS best choice for preserving outliers, PCA for variance, & T-SNE for clusters"

MOOC(Massive Open Online Courses)

git and version control


data management

Automate your workflow, open science and reproducible research

Automation wins in the long run.

STEP 6 is usually missing!

The pic was downloaded from

Workflow languages


I am using snakemake and so far is very happy about it!


Reproducible research

As an early adopter of the Figshare repository, I came up with a strategy that serves both our open-science and our reproducibility goals, and also helps with this problem: for the main results in any new paper, we would share the data, plotting script and figure under a CC-BY license, by first uploading them to Figshare.

Survival curve

Organize research for a group

  • slack:A messaging app for teams.
  • Ryver.
  • Trello lets you work more collaboratively and get more done.


CRISPR related

vector arts for life sciences

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